The Phospho-c-Myc-S62 Monoclonal Antibody (CABP0989) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically recognizes the phosphorylated form of c-Myc at serine 62, a critical post-translational modification that affects its stability and activity. Raised in rabbits, this antibody has high sensitivity and specificity for human samples, making it ideal for use in Western blot and immunohistochemistry applications. By targeting the phosphorylated form of c-Myc, researchers can gain insights into the signaling pathways and functions regulated by this protein in various biological processes, including cancer development and progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-c-Myc-S62 Monoclonal Antibody
SKU:
CABP0989
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1533
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PLSP S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MRTL, MYCC, c-Myc, bHLHe39, Phospho-c-Myc-S62
Positive Sample:
293T treated with Forskolin, NIH/3T3 treated with Forskolin
Cellular Localization:
Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
51kDa
Observed MW:
57-65kDa
This gene is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.
Purification Method
Affinity purification
Gene ID
4609
RRID
AB_2863883
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-c-Myc-S62 Rabbit mAb (CABP0989) at 1:1000 dilution. 293T cells were treated with Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
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