The Phospho-CDK1-T14 Antibody (CABP0015) is a high-quality antibody developed for reliable detection and analysis of target proteins. CDK1, also known as cyclin-dependent kinase 1, is a key player in cell division and is regulated by phosphorylation at threonine 14 (T14) during mitosis. This antibody, raised in rabbits, is highly specific for the phosphorylated form of CDK1 and is validated for use in Western blot applications.The Phospho-CDK1 (T14) antibody binds specifically to the phosphorylated form of CDK1, allowing for precise detection and analysis of CDK1 activity in various cell types. This antibody is essential for studying the role of CDK1 phosphorylation in cell cycle progression and mitotic events, making it an ideal tool for research in cell biology, cancer biology, and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-CDK1-T14 Antibody
SKU:
CABP0015
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
EGTY G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC2, CDC28A, P34CDC2, Phospho-CDK1-T14
Positive Sample:
HeLa treated with Hydroxyurea
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
983
RRID
AB_2770976
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T14 Rabbit pAb (CABP0015) at 1:500 dilution. HeLa cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
