The Phospho-CDK1-T161 Antibody (CABP0324) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the phosphorylated form of CDK1 at threonine 161, a key regulatory site for the activity of this important cell cycle regulator.CDK1 is a critical kinase involved in the control of cell division and plays a vital role in regulating the G2/M transition. Phosphorylation of CDK1 at threonine 161 is known to be essential for the activation of the kinase and the onset of mitosis. This antibody enables researchers to detect and analyze the phosphorylation status of CDK1 in various cell types, providing valuable insights into the mechanisms controlling cell cycle progression.
This antibody is validated for use in WB, IHC-P, IP, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-CDK1-T161 Antibody
SKU:
CABP0324
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VYTH E
Tested Applications:
WBIHC-PIPELISAIF-P
Recommended Dilution:
WB
1:100 - 1:500
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
IF-P
1:50 - 1:200
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC2, CDC28A, P34CDC2, Phospho-CDK1-T161
Positive Sample:
HeLa treated with EGF
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Centrosome, Cytoskeleton, Microtubule Organizing Center, Spindle.
Calculated MW:
34kDa
Observed MW:
34kDa
The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
983
RRID
AB_2770977
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at 1:400 dilution. HeLa cells were treated with EGF. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of Rat testis tissue using Phospho-CDK1-T161 Rabbit pAb (CABP0324) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-CDK1-T161 pAb (CABP0324). Western blot was performed from the immunoprecipitate using Phospho-CDK1-T161 pAb (CABP0324) at a dilution of 1:1000. HeLa cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
