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Anti-Phospho-CDK4-T172 Antibody (CABP0593)

SKU:
CABP0593
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€329 - €419
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Description

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Antibody Name:Anti-Phospho-CDK4-T172 Antibody
Antibody SKU:CABP0593
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3
Immunogen:A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ALTP V
Gene ID:1019
Uniprot:P11802
Cellular Location:Cytoplasm, Membrane, Nucleus
Calculated MW:20kDa/33kDa
Observed MW:33KDa
Anti-Phospho-CDK4-T172 Antibody (CABP0593)Western blot analysis of extracts of NIH/3T3 cells, using Anti-Phospho-CDK4-T172 Antibody (CABP0593) at 1:1000 dilution or CDK4 antibody (CAB0366).NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Synonyms:CDK4, CMM3, PSK-J3
Background:The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
UniProt Protein Function:CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.
UniProt Protein Details:

Protein type:EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: nucleoplasm; transcription factor complex; nuclear membrane; tight junction; perinuclear region of cytoplasm; nucleolus; cyclin-dependent protein kinase holoenzyme complex; nucleus; chromatin; cytosol

Molecular Function:protein binding; cyclin binding; cyclin-dependent protein kinase activity; protein complex binding; cyclin-dependent protein kinase regulator activity; ATP binding

Biological Process: circadian rhythm; lens development in camera-type eye; response to drug; establishment and/or maintenance of chromatin architecture; organ regeneration; positive regulation of cell size; positive regulation of translation; positive regulation of apoptosis; response to toxin; response to testosterone stimulus; signal transduction; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; response to hyperoxia; regulation of gene expression; cell division; response to lead ion; positive regulation of cell proliferation; mitotic cell cycle; regulation of protein kinase activity; G1/S transition of mitotic cell cycle

Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3

NCBI Summary:The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:P11802
NCBI GenInfo Identifier:1168867
NCBI Gene ID:1019
NCBI Accession:P11802.2
UniProt Secondary Accession:P11802,O00576, Q6FG61, B2R9A0, B4DNF9,
UniProt Related Accession:P11802
Molecular Weight:Calculated MW: 20kDa/33kDaObserved MW: 34kDa
NCBI Full Name:Cyclin-dependent kinase 4
NCBI Synonym Full Names:cyclin-dependent kinase 4
NCBI Official Symbol:CDK4  
NCBI Official Synonym Symbols:CMM3; PSK-J3  
NCBI Protein Information:cyclin-dependent kinase 4; cell division protein kinase 4
UniProt Protein Name:Cyclin-dependent kinase 4
UniProt Synonym Protein Names:Cell division protein kinase 4; PSK-J3
Protein Family:Cyclin-dependent kinase
UniProt Gene Name:CDK4  
UniProt Entry Name:CDK4_HUMAN

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