Anti-Phospho-CHUK-S176 Antibody (CABP0505)
- SKU:
- CABP0505
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Phospho-CHUK-S176 Antibody |
| Antibody SKU: | CABP0505 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S176 of human IKKAlpha (NP_001269.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, C6 |
| Immunogen: | A synthetic phosphorylated peptide around S176 of human IKKAlpha (NP_001269.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QGSL C |
| Gene ID: | 1147 |
| Uniprot: | O15111 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 84kDa |
| Observed MW: | 84kDa |
 | Western blot analysis of extracts of C6 cells, using Phospho-IKKa-S176 antibody (CABP0505) at 1:1000 dilution.C6 cells were treated by TNF-a (20 ng/mL) at 37'C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of HeLa cells, using Phospho-IKKa-S176 pAb (CABP0505) at 1:1000 dilution or IKK alpha antibody (CAB0423).HeLa cells were treated by TNF-a (20 ng/mL) at 37'C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| Synonyms: | CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, IKK alpha |
| Background: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. |
| UniProt Protein Function: | IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.10; Other group; IKK family Chromosomal Location of Human Ortholog: 10q24-q25 Cellular Component: nucleoplasm; internal side of plasma membrane; intracellular membrane-bound organelle; cytoplasm; IkappaB kinase complex; cytosol Molecular Function:protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; ATP binding; protein kinase activity Biological Process: lactation; skeletal muscle contraction; I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; response to toxin; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; osteoclast differentiation; T cell receptor signaling pathway; protein amino acid phosphorylation; Rho protein signal transduction; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of interferon type I production; morphogenesis of an epithelial sheet; inflammatory response; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; response to drug; anatomical structure morphogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; response to virus; MyD88-independent toll-like receptor signaling pathway; mammary gland epithelial cell proliferation; response to amino acid stimulus; odontogenesis of dentine-containing teeth; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to hydroperoxide; toll-like receptor signaling pathway; striated muscle cell differentiation; innate immune response; immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation Disease: Cocoon Syndrome |
| NCBI Summary: | This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O15111 |
| NCBI GenInfo Identifier: | 317373368 |
| NCBI Gene ID: | 1147 |
| NCBI Accession: | O15111.2 |
| UniProt Secondary Accession: | O15111,O14666, Q13132, Q5W0I4, Q92467, |
| UniProt Related Accession: | O15111 |
| Molecular Weight: | Calculated MW: 84kDaObserved MW: 85kDa |
| NCBI Full Name: | Inhibitor of nuclear factor kappa-B kinase subunit alpha |
| NCBI Synonym Full Names: | conserved helix-loop-helix ubiquitous kinase |
| NCBI Official Symbol: | CHUKÂ Â |
| NCBI Official Synonym Symbols: | IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha  |
| NCBI Protein Information: | inhibitor of nuclear factor kappa-B kinase subunit alpha; TCF-16; IKK-a kinase; I-kappa-B kinase 1; I-kappa-B kinase-alpha; transcription factor 16; IkB kinase alpha subunit; Nuclear factor NFkappaB inhibitor kinase alpha |
| UniProt Protein Name: | Inhibitor of nuclear factor kappa-B kinase subunit alpha |
| UniProt Synonym Protein Names: | Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16 |
| Protein Family: | Inhibitor of nuclear factor kappa-B kinase |
| UniProt Gene Name: | CHUKÂ Â |
| UniProt Entry Name: | IKKA_HUMAN |
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