Anti-DDIT3/CHOP Antibody (CAB0221)

Antibody Name:	Anti-DDIT3 / CHOP Antibody
Antibody SKU:	CAB0221
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 / CHOP (NP_004074.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, C2C12, C6

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human DDIT3 / CHOP (NP_004074.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GRTR KRKQ SGHS PARA GKQR MKEK EQEN ERKV AQLA EENE RLKQ EIER LTRE VEAT RRAL IDRM VNLH QA
Gene ID:	1649
Uniprot:	P35638
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	19kDa/21kDa
Observed MW:	25kDa

Synonyms:

DDIT3, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153, C/EBPzeta, DDIT3 / CHOP

Background:

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

UniProt Protein Function:	CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.
UniProt Protein Details:	Protein type:Autophagy; Oncoprotein; Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 12q13.1-q13.2 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:cAMP response element binding protein binding; DNA binding; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity; transcription factor binding Biological Process: cell cycle arrest; cell redox homeostasis; inhibition of CREB transcription factor; mRNA transcription from RNA polymerase II promoter; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of interleukin-8 production; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription in response to stress; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to unfolded protein Disease: Myxoid Liposarcoma
NCBI Summary:	This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]
UniProt Code:	P35638
NCBI GenInfo Identifier:	544022
NCBI Gene ID:	1649
NCBI Accession:	P35638.1
UniProt Secondary Accession:	P35638,F8VS99,
UniProt Related Accession:	P35638
Molecular Weight:	21,700 Da
NCBI Full Name:	DNA damage-inducible transcript 3 protein
NCBI Synonym Full Names:	DNA damage inducible transcript 3
NCBI Official Symbol:	DDIT3
NCBI Official Synonym Symbols:	CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
NCBI Protein Information:	DNA damage-inducible transcript 3 protein
UniProt Protein Name:	DNA damage-inducible transcript 3 protein
UniProt Synonym Protein Names:	C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153
Protein Family:	DNA damage-inducible transcript 3 protein
UniProt Gene Name:	DDIT3
UniProt Entry Name:	DDIT3_HUMAN

	Western blot analysis of extracts of various cell lines, using DDIT3 / CHOP antibody (CAB0221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat testis using DDIT3 / CHOP antibody (CAB0221) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using DDIT3 / CHOP antibody (CAB0221) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using DDIT3 / CHOP antibody (CAB0221) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using DDIT3 / CHOP antibody (CAB0221) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-DDIT3 / CHOP Antibody (CAB0854)	Human GADD153 / DDIT3 ELISA Kit
Anti-DDIT3 / CHOP Antibody (CAB11346)	Human DNA damage-inducible transcript 3 protein (DDIT3) ELISA Kit
Anti-DDIT3 / CHOP Antibody (CAB6504)	GADD153 Colorimetric Cell-Based ELISA
Phospho-DDIT3 (S30) Antibody (PACO03564)
DDIT3 Antibody (PACO06350)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)