Anti-Phospho-EEF2K-S366 pAb Antibody (CABP0845)
- SKU:
- CABP0845
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Phospho-EEF2K-S366 Antibody |
| Antibody SKU: | CABP0845 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, NIH/3T3, C6 |
| Immunogen: | A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TLSG S |
| Gene ID: | 29904 |
| Uniprot: | O00418 |
| Cellular Location: | |
| Calculated MW: | 82kDa |
| Observed MW: | 110kDa |
 | Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (CABP0845) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
 | Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody (CABP0845) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour or PMA/TPA (200 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of HeLa cells, using Anti-Phospho-EEF2K-S366 pAb (CABP0845) at 1:1000 dilution or EEF2K antibody (CAB5404).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
| Synonyms: | EEF2K, HSU93850, eEF-2K |
| Background: | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. |
| UniProt Protein Function: | EEF2K: an atypical protein kinase of the Alpha family. Dependent on Ca2 ions and calmodulin. May play a key role linking cellular energy status and the inhibition of protein synthesis, a major consumer of metabolic energy. Phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation eEF2K is phosphorylated and activated by AMPK in response to elevated AMP levels, which increase during cellular stress and starvation. Regulated in response to a range of stimuli including TNF-alpha. Overexpressed in breast cancer cell lines and tumors. Activated in rat glioblastoma, and growth was inhibited by the drug Rottlerin. Activity induced in ischemic heart muscle |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Translation; Kinase, protein; Protein kinase, atypical; EC 2.7.11.20; ATYPICAL group; Alpha family; eEF2K subfamily Chromosomal Location of Human Ortholog: 16p12.2 Cellular Component: cytoplasm; cytosol Molecular Function:calmodulin binding; elongation factor-2 kinase activity; translation factor activity, nucleic acid binding; calcium ion binding; ATP binding; protein kinase activity Biological Process: translational elongation; regulation of protein amino acid autophosphorylation; protein amino acid autophosphorylation; insulin receptor signaling pathway |
| NCBI Summary: | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00418 |
| NCBI GenInfo Identifier: | 254763289 |
| NCBI Gene ID: | 29904 |
| NCBI Accession: | O00418.2 |
| UniProt Secondary Accession: | O00418,Q8N588, |
| UniProt Related Accession: | O00418 |
| Molecular Weight: | 725 |
| NCBI Full Name: | Eukaryotic elongation factor 2 kinase |
| NCBI Synonym Full Names: | eukaryotic elongation factor 2 kinase |
| NCBI Official Symbol: | EEF2KÂ Â |
| NCBI Official Synonym Symbols: | eEF-2K; HSU93850Â Â |
| NCBI Protein Information: | eukaryotic elongation factor 2 kinase; eEF-2 kinase; elongation factor-2 kinase; eukaroytic elongation factor 2 kinase; eukaryotic elongation factor-2 kinase; calmodulin-dependent protein kinase III; calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase |
| UniProt Protein Name: | Eukaryotic elongation factor 2 kinase |
| UniProt Synonym Protein Names: | Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase |
| Protein Family: | Eukaryotic elongation factor 2 kinase |
| UniProt Gene Name: | EEF2KÂ Â |
| UniProt Entry Name: | EF2K_HUMAN |
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