Anti-Phospho-EIF4EBP1-S65 Antibody (CABP0032)

SKU:
CABP0032
€99 - €319

Description

Technical Manual

Anti-Phospho-EIF4EBP1-S65 Antibody

Product Name:Anti-Phospho-EIF4EBP1-S65 Antibody
Product SKU:CABP0032
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Immunogen:A synthetic phosphorylated peptide around S65 of human EIF4EBP1 (NP_004086.1).
Sequence:

RNSPV

Gene ID:1978
Swiss Prot:Q13541
Synonyms:4E-BP1; 4EBP1; BP-1; PHAS-I; EIF4EBP1
Calculated MW:12kDa
Observed MW:18kDa
Reactivity:Human
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:100
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunoprecipitation
Positive Samples:293T, HeLa
Cellular Location:

Product Images

Anti-Phospho-EIF4EBP1-S65 Antibody (CABP0032)

Western blot analysis of extracts of HeLa cells, using Phospho-EIF4EBP1-S65 antibody (CABP0032) at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Uniprot Information

UniProt Protein Function:4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.
UniProt Protein Details:

Protein type:Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function:protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; TOR signaling pathway; response to ethanol; cellular protein metabolic process; translation; translational initiation; insulin receptor signaling pathway; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development

NCBI Summary:This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Code:Q13541
NCBI GenInfo Identifier:34921508
NCBI Gene ID:1978
NCBI Accession:Q13541.3
UniProt Secondary Accession:Q13541,Q6IBN3, B2R502, D3DSW8,
UniProt Related Accession:Q13541
Molecular Weight:118
NCBI Full Name:Eukaryotic translation initiation factor 4E-binding protein 1
NCBI Synonym Full Names:eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol:EIF4EBP1
NCBI Official Synonym Symbols:BP-1; 4EBP1; 4E-BP1; PHAS-I
NCBI Protein Information:eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
UniProt Protein Name:Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names:Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Protein Family:Eukaryotic translation initiation factor 4E-binding protein
UniProt Gene Name:EIF4EBP1
UniProt Entry Name:4EBP1_HUMAN
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Additional Information

Product type:
Antibody
Application:
WB
Application:
IP
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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