Western blot analysis of extracts from MCF7 cells, using Phospho-ErbB3/HER3-Y1289 Rabbit mAb (CABP1415) at 1:1000 dilution. MCF7 cells were treated by neuregulin at 37℃ for 15 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-ErbB3/HER3-Y1289 Monoclonal Antibody (CABP1415) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed and validated by AssayGenie, specifically targets the Y1289 phosphorylation site on the ERBB3 (HER3) protein.ERBB3 (HER3) is a member of the epidermal growth factor receptor (EGFR) family and plays a crucial role in cell proliferation, survival, and differentiation. Phosphorylation at the Y1289 site is known to be involved in the activation of downstream signaling pathways associated with cancer progression and drug resistance.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-ErbB3/HER3-Y1289 Monoclonal Antibody
SKU:
CABP1415
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58831
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
LGYE Y
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
148kDa
Observed MW:
185kDa/200kDa
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
Purification Method
Affinity purification
Gene ID
2065
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from MCF7 cells, using Phospho-ErbB3/HER3-Y1289 Rabbit mAb (CABP1415) at 1:1000 dilution.MCF7 cells were treated with neuregulin at 37℃ for 15 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 45s.
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