Anti-Phospho-FoxO1-S256 Antibody (CABP0172)
- SKU:
- CABP0172
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-Phospho-FoxO1-S256 Antibody |
| Antibody SKU: | CABP0172 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around S256 of human FoxO1 (NP_002006.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human |
| Positive Samples: | 293T, HeLa |
| Immunogen: | A synthetic phosphorylated peptide around S256 of human FoxO1 (NP_002006.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AASM D |
| Gene ID: | 2308 |
| Uniprot: | Q12778 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 69kDa |
| Observed MW: | 100kDa |
 | Western blot analysis of extracts of 293T cells, using Phospho-FoxO1-S256 antibody (CABP0172) .293T were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of extracts of HeLa cells, using Anti-Phospho-FoxO1-S256 Antibody (CABP0172) at 1:1000 dilution or FOXO1 antibody (CAB13862).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37'C for 1 hour or treated by 10% FBS at 37'C for 30 minutes after serum-starvation overnight.. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 2s. |
| Synonyms: | FKH1, FKHR, FOXO1A, FOXO1 |
| Background: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. |
| UniProt Protein Function: | FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Oncoprotein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 13q14.1 Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus Molecular Function:beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding Biological Process: anatomical structure morphogenesis; apoptosis; autophagy; blood vessel development; cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; epidermal growth factor receptor signaling pathway; fat cell differentiation; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of gluconeogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation; transcription, DNA-dependent Disease: Rhabdomyosarcoma 2 |
| NCBI Summary: | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12778 |
| NCBI GenInfo Identifier: | 116241368 |
| NCBI Gene ID: | 2308 |
| NCBI Accession: | Q12778.2 |
| UniProt Secondary Accession: | Q12778,O43523, Q5VYC7, Q6NSK6, |
| UniProt Related Accession: | Q12778 |
| Molecular Weight: | |
| NCBI Full Name: | Forkhead box protein O1 |
| NCBI Synonym Full Names: | forkhead box O1 |
| NCBI Official Symbol: | FOXO1Â Â |
| NCBI Official Synonym Symbols: | FKH1; FKHR; FOXO1AÂ Â |
| NCBI Protein Information: | forkhead box protein O1 |
| UniProt Protein Name: | Forkhead box protein O1 |
| UniProt Synonym Protein Names: | Forkhead box protein O1A; Forkhead in rhabdomyosarcoma |
| Protein Family: | Foxo1-corepressor |
| UniProt Gene Name: | FOXO1Â Â |
| UniProt Entry Name: | FOXO1_HUMAN |
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