The Phospho-HSP27/HSPB1-S15 Antibody (CABP0040) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to the phosphorylated form of HSPB1 at serine 15 and is validated for use in Western blot applications. By detecting and binding to phosphorylated HSPB1, researchers can investigate its role in cellular signaling pathways and stress response mechanisms.HSPB1, also known as heat shock protein 27 (Hsp27), is a key player in protecting cells from stress-induced damage, making it a potential therapeutic target for diseases characterized by cellular stress, such as neurodegenerative disorders and cardiovascular diseases.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-HSP27/HSPB1-S15 Antibody
SKU:
CABP0040
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GPSW D
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Purification Method
Affinity purification
Gene ID
3315
RRID
AB_2771183
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit pAb (CABP0040) at 1:600 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-HSP27/HSPB1-S15 pAb (CABP0040). Western blot was performed from the immunoprecipitate using Phospho-HSP27/HSPB1-S15 pAb (CABP0040) at a dilution of 1:1000. HeLa cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.
