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Anti-Phospho-IRE1alpha-S724 pAb Antibody (CABP0878)

SKU:
CABP0878
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-Phospho-IRE1alpha-S724 pAb Antibody CABP0878
  • Cell Death Antibodies 2 Anti-Phospho-IRE1alpha-S724 pAb Antibody CABP0878
  • Cell Death Antibodies 2 Anti-Phospho-IRE1alpha-S724 pAb Antibody CABP0878
€329 - €419
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Description

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Antibody Name:Anti-Phospho-IRE1alpha-S724 Antibody
Antibody SKU:CABP0878
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding s724 of human IRE1alpha.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3
Immunogen:A phospho specific peptide corresponding to residues surrounding s724 of human IRE1alpha.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2081
Uniprot:O75460
Cellular Location:Endoplasmic reticulum membrane, Single-pass type I membrane protein
Calculated MW:6kDa/109kDa
Observed MW:130KDa
Anti-Phospho-IRE1alpha-S724 pAb (CABP0878)Western blot analysis of extracts of NIH/3T3 cells, using Phospho-IRE1alpha-S724 antibody (CABP0878) at 1:500 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Phospho-IRE1alpha-S724 pAb (CABP0878)Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-IRE1alpha(ser724) antibody (CABP0878) at dilution of 1:100 (40x lens).
Anti-Phospho-IRE1alpha-S724 pAb (CABP0878)Immunohistochemistry of paraffin-embedded rat spleen using Phospho-IRE1alpha(ser724) antibody (CABP0878) at dilution of 1:100 (40x lens).
Synonyms:ERN1, IRE1, IRE1P, IRE1a, hIRE1p
Background:The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals.
NCBI Summary:This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
UniProt Code:O75460
NCBI GenInfo Identifier:193806335
NCBI Gene ID:2081
NCBI Accession:O75460.2
UniProt Secondary Accession:O75460,Q76MJ5,
UniProt Related Accession:O75460
Molecular Weight:Observed: 110 kDaPredicted: 110 kDa
NCBI Full Name:Serine/threonine-protein kinase/endoribonuclease IRE1
NCBI Synonym Full Names:endoplasmic reticulum to nucleus signaling 1
NCBI Official Symbol:ERN1  
NCBI Official Synonym Symbols:IRE1; IRE1P; IRE1a; hIRE1p  
NCBI Protein Information:serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Protein Name:Serine/threonine-protein kinase/endoribonuclease IRE1
UniProt Synonym Protein Names:Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a
Protein Family:Serine/threonine-protein kinase/endoribonuclease
UniProt Gene Name:ERN1  
UniProt Entry Name:ERN1_HUMAN

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