Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody (CABP0631)
- SKU:
- CABP0631
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- IF
- IP
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Phospho-JNK1/2/3-T183/T183/T221 mAb Antibody |
| Antibody SKU: | CABP0631 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3 |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, C6 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T183/T183/T221 of human JNK1/2/3 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 5599/5601/5602 |
| Uniprot: | P45983/P45984/P53779 |
| Cellular Location: | |
| Calculated MW: | 35kDa/44kDa/48kDa/27kDa/52kDa |
| Observed MW: | 46KDa/56KDa |
 | Western blot analysis of extracts of various cell lines, using Phospho-JNK1/2/3-T183/T183/T221 Rabbit mAb (CABP0631) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight.C6 cells were treated by Anisomycin (25 ug/mL) at 37'C for 20 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human appendix using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using Phospho-JNK1/2/3-T183/T183/T221 antibody (CABP0631).NIH-3T3 cells were treated by Anisomycin (25 ug/mL) at 37'C for 30 minutes after serum-starvation overnight. Blue: DAPI for nuclear staining. |
| Synonyms: | JNK1/JNK2/JNK3 |
| Background: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016] |
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