The Phospho-VEGF Receptor 2-Y1175 Antibody (CABP0382) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to the phosphorylated form of KDR at tyrosine 1175 and is suitable for use in Western blot applications. KDR is a key receptor involved in angiogenesis, playing a crucial role in the formation of new blood vessels. Phosphorylation of KDR at tyrosine 1175 is a critical event in the activation of downstream signaling pathways that regulate angiogenesis. The Phospho-KDR (Y1175) Polyclonal Antibody enables researchers to detect and analyze the phosphorylation status of KDR in various cell types, making it a valuable tool for studies in cancer research, cardiovascular disease, and other angiogenesis-related disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-VEGF Receptor 2-Y1175 Antibody
SKU:
CABP0382
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KDYI V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Junction, Cell Membrane, Cytoplasm, Cytoplasmic Vesicle, Early Endosome, Endoplasmic Reticulum, Nucleus, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
152kDa
Observed MW:
230kDa
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Purification Method
Affinity purification
Gene ID
3791
RRID
AB_2771247
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-VEGF Receptor 2-Y1175 Rabbit pAb (CABP0382) at 1:500 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
