The Phospho-MAP2K6-S207/MAP2K3-S189 Antibody (CABP0081) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting phosphorylated forms of MAP2K6 at serine 207 and MAP2K3 at serine 189 in human samples.The phosphorylation of MAP2K6 and MAP2K3 is known to regulate their activity, leading to downstream effects on cell growth, differentiation, and apoptosis. This antibody is validated for use in Western blot applications, allowing for the detection and analysis of phosphorylated MAP2K6 and MAP2K3 in different cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-MAP2K6-S207/MAP2K3-S189 Antibody
SKU:
CABP0081
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VDSV A
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MAP2K3/MAP2K6, Phospho-MAP2K6-S207/MAP2K3-S189
Positive Sample:
NIH/3T3 treated with PMA
Cellular Localization:
Cytosol, Nucleoplasm.
Calculated MW:
36kDa/39kDa/31kDa/37kDa
Observed MW:
36kDa
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
5606 5608
RRID
AB_2771296
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MAP2K6-S207/MAP2K3-S189 Rabbit pAb (CABP0081) at 1:500 dilution. NIH/3T3 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
