The Phospho-MAP3K5-S83 Antibody (CABP0059) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the phosphorylated form of MAP3K5 at Serine 83 and has been validated for use in Western blot applications. It enables the detection and analysis of MAP3K5 phosphorylation in various cell types, making it ideal for investigations in immunology, cancer research, and signal transduction.MAP3K5, also known as apoptosis signal-regulating kinase 1 (ASK1), plays a crucial role in regulating cell survival, apoptosis, and inflammation in response to extracellular stress signals. Its activation through phosphorylation at Serine 83 is associated with various cellular processes, including immune response modulation and tumor development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-MAP3K5-S83 Antibody
SKU:
CABP0059
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
GSSV G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASK1, MEKK5, MAPKKK5, Phospho-MAP3K5-S83
Positive Sample:
K-562 treated with ATP
Cellular Localization:
Cytoplasm, Endoplasmic Reticulum.
Calculated MW:
155kDa
Observed MW:
155kDa
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Purification Method
Affinity purification
Gene ID
4217
RRID
AB_2771300
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-MAP3K5-S83 Rabbit pAb (CABP0059) at 1:1000 dilution. K562 cell lysate were treated with CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour, treated with TNF-α (20ng/mL) for 30 minutes after serum-starvation overnight or treated with ATP (5mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
