The Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Monoclonal Antibody (CABP0974) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to detect phosphorylation at threonine 202 and tyrosine 204 in ERK1, and threonine 185 and tyrosine 187 in ERK2, making it a valuable resource for investigating the activation of these kinases in cell signaling pathways.The antibody, developed using rabbit monoclonal technology, offers high specificity and sensitivity for detecting phosphorylated ERK1 and ERK2 in a variety of cell types and experimental conditions.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
5595 5594
RRID
AB_2863871
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (CABP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (CAB4782) at1:3000 dilution (lower) incubated overnight at 4℃. 293T cells were treated with PMA (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (). Exposure time: 10s.
Western blot analysis of lysates from C6 cells using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (CABP0974) at 1:1000 dilution (upper) or ERK1/2 Rabbit mAb (CAB4782) at1:3000 dilution (lower) incubated overnight at 4℃. C6 cells were treated with PMA (200 nM) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (). Exposure time: 45s.
Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit mAb (CABP0974) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
