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Anti-Phospho-MYL9-S19 pAb Antibody (CABP0872)

SKU:
CABP0872
Product Type:
Antibody
Applications:
WB
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€329 - €419
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Description

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Antibody Name:Anti-Phospho-MYL9-S19 Antibody
Antibody SKU:CABP0872
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S19 of human MYL9 (NP_006088.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:C6
Immunogen:A synthetic phosphorylated peptide around S19 of human MYL9 (NP_006088.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RATS N
Gene ID:10398
Uniprot:P24844
Cellular Location:
Calculated MW:13kDa/19kDa
Observed MW:19kDa
Anti-Phospho-MYL9-S19 pAb (CABP0872)Western blot analysis of extracts of C6 cells, using Phospho-MYL9-S19 antibody (CABP0872) at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:MYL9, LC20, MLC-2C, MLC2, MRLC1, MYRL2
Background:Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:MRLC1: Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. Myosin is an hexamer of 2 heavy chains and 4 light chains. Smooth muscle tissues and in some, but not all, nonmuscle cells. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Contractile; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: cytosol; muscle myosin complex; stress fiber; Z disc

Molecular Function:calcium ion binding; myosin heavy chain binding; structural constituent of muscle

Biological Process: axon guidance; ephrin receptor signaling pathway; muscle contraction; regulation of muscle contraction; small GTPase mediated signal transduction

NCBI Summary:Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P24844
NCBI GenInfo Identifier:20141521
NCBI Gene ID:10398
NCBI Accession:P24844.4
UniProt Secondary Accession:P24844,Q9BQL9, Q9BUF9, Q9H136, E1P5T6,
UniProt Related Accession:P24844
Molecular Weight:13,866 Da
NCBI Full Name:Myosin regulatory light polypeptide 9
NCBI Synonym Full Names:myosin light chain 9
NCBI Official Symbol:MYL9  
NCBI Official Synonym Symbols:LC20; MLC2; MRLC1; MYRL2; MLC-2C  
NCBI Protein Information:myosin regulatory light polypeptide 9
UniProt Protein Name:Myosin regulatory light polypeptide 9
UniProt Synonym Protein Names:20 kDa myosin light chain; LC20; MLC-2C; Myosin RLC; Myosin regulatory light chain 2, smooth muscle isoform; Myosin regulatory light chain 9; Myosin regulatory light chain MRLC1
Protein Family:Myosin regulatory light polypeptide
UniProt Gene Name:MYL9  
UniProt Entry Name:MYL9_HUMAN

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