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Phospho-NEDD4L-S448 Rabbit Polyclonal Antibody (CABP0843)

SKU:
CABP0843
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Developmental Biology
  • Developmental Biology Anti-Phospho-NEDD4L-S448 pAb Antibody CABP0843
  • Developmental Biology Anti-Phospho-NEDD4L-S448 pAb Antibody CABP0843
€419
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-NEDD4L-S448 Rabbit Polyclonal Antibody
SKU:CABP0843
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S448 of human NEDD4L (NP_001138439.1).
Sequence:SLSS P
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:RSP5; PVNH7; NEDD4-2; NEDD4.2; hNEDD4-2; Phospho-NEDD4L-S448
Positive Sample:293T,NIH/3T3,C6
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:112kDa
Observed MW:100kDa/130kDa

This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Purification Method:Affinity purification
Gene ID:23327
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Phospho-NEDD4L-S448 Rabbit pAb (CABP0843) at 1:1000 dilution. NIH 3T3 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour. C6 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

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