Western blot analysis of 293T, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (CABP1502) at 1:1000 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-p38 MAPK-T180/Y182 Monoclonal Antibody (CABP1502) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using hybridoma technology, exhibits high specificity and sensitivity towards the phosphorylated p38 MAPK protein in human samples, making it ideal for use in Western blot and immunohistochemistry applications.Phosphorylation of p38 MAPK at threonine 180 and tyrosine 182 residues is crucial for its activation and downstream signaling cascades in response to various stimuli, including environmental stresses, cytokines, and growth factors. The Phospho p38 MAPK (T180/Y182) Monoclonal Antibody enables researchers to detect and analyze phosphorylated p38 MAPK levels in different cell types, providing valuable insights into the regulation of cellular processes such as cell proliferation, differentiation, and apoptosis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-p38 MAPK-T180/Y182 Monoclonal Antibody
SKU:
CABP1502
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC62666
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
MTGY VA
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
293T treated with UV, NIH/3T3 treated with UV, C6 treated with Anisomycin
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
41kDa
Observed MW:
40kDa
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Purification Method
Affinity purification
Gene ID
5600 6300 5603 1432
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (CABP1502) at 1:1000 dilution. 293T cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p38 MAPK-T180/Y182 Rabbit mAb (CABP1502) at 1:1000 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
