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Anti-Phospho-PKC (pan) (beta-II Ser660) Antibody (CABP0495)

SKU:
CABP0495
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€329 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Phospho-PKC (pan) (beta-II Ser660) Antibody
Antibody SKU:CABP0495
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) BetaII
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, 293T+PMA
Immunogen:A phospho specific peptide corresponding to residues surrounding Ser660 of human PKC (pan) BetaII
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:5578/5579/5580/5581/5583/5588
Uniprot:P17252/P05771/Q05655/Q02156/P24723/Q04759
Cellular Location:
Calculated MW:59kDa/67kDa/74- 83kDa
Observed MW:95kDa
Anti-Phospho-PKC (pan) (beta-II Ser660) Antibody (CABP0495)Western blot analysis of extracts of 293T cells, using Phospho-PKC (pan) (beta II Ser660) pAb (CABP0495) at 1:1000 dilution or PRKCQ antibody (CAB5834).293T cells were treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Synonyms:PRKCA/PRKCB/PRKCD/PRKCE/PRKCH/PRKCQ
Background:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

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