The Phospho-PLK1-T210 Monoclonal Antibody (CABP1025) is a high-quality antibody developed for reliable detection and analysis of target proteins. PLK1 is a key regulator of cell cycle progression and has been implicated in various cancer types. This antibody, raised in rabbits, is highly specific for detecting phosphorylated PLK1 at T210 in human samples and is validated for use in Western blot applications.Phosphorylation of PLK1 at T210 is known to be a critical regulatory event in its activation and function, particularly in promoting cell division and progression through mitosis. Understanding the phosphorylation status of PLK1 can provide important insights into its role in cancer development and progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PLK1-T210 Monoclonal Antibody
SKU:
CABP1025
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1570
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
KKTL C
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy.
Purification Method
Affinity purification
Gene ID
5347
RRID
AB_2863909
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HT-29 cells, using Phospho-PLK1-T210 (CABP1025) at 1:1000 dilution. HT-29 cells were treated with nocodazole (100 ng/mL) at 37℃ for 16 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
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