The Phospho-Rac1/2/3-S71 Antibody (CABP0086) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for detecting phosphorylation of Rac1 at serine 71 in human samples, making it a valuable tool for investigating Rac1 signaling in various cellular processes.Rac1 is a member of the Rho family of GTPases and plays a crucial role in cellular migration, proliferation, and cytoskeletal dynamics. Phosphorylation of Rac1 at serine 71 has been shown to regulate its activity and function in different cellular contexts, including cancer progression, cell adhesion, and cell motility.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-Rac1/2/3-S71 Antibody
SKU:
CABP0086
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RPLS YP
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5879 5880 5881
RRID
AB_2771488
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (CABP0086) at 1:1000 dilution. A-431 cells were treated with EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
