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Western blot analysis of HeLa, using Phospho-RB-S780 Rabbit mAb (CABP1419) at 1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

Phospho-RB-S780 Monoclonal Antibody (CABP1419)

Datasheet MSDS
SKU CABP1419
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Rat
Host Species Rabbit
Synonyms RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1, Phospho-RB-S780
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

Phospho-RB-S780 Monoclonal Antibody (CABP1419)

The Phospho-RB-S780 Monoclonal Antibody (CABP1419) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through a rigorous process in mouse cells, is highly specific for detecting phosphorylated Rb in human samples and is validated for use in Western blot applications.The phosphorylation of Rb at serine 780 is known to regulate cell cycle progression and is implicated in various diseases, including cancer. This antibody enables precise detection and analysis of phosphorylated Rb in different cell types, making it a valuable asset for studies in cell biology, cancer research, and drug development.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.

Product Name:Phospho-RB-S780 Monoclonal Antibody
SKU:CABP1419
Size:20μL, 100μL
Reactivity:Human, Rat
Clone Number:ARC52461
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:WB ELISA
Recommended Dilution:
WB1:2000 - 1:20000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1, Phospho-RB-S780
Positive Sample:HeLa treated with Calyculin A, C6 treated with Calyculin A
Cellular Localization:Nucleus.
Calculated MW:106kDa
Observed MW:110kDa

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Purification MethodAffinity purification
Gene ID5925
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from HeLa cells, using Phospho-RB-S780 Rabbit mAb (CABP1419) at 1:19000 dilution. HeLa cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Product ImageWestern blot analysis of lysates from C6 cells, using Phospho-RB-S780 Rabbit mAb (CABP1419) at 1:19000 dilution. C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
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