Description
Phospho-RPA2-S4/S8 Polyclonal Antibody (CABP1102)
The Phospho-RPA2-S4/S8 Polyclonal Antibody (CABP1102) is a high-quality antibody developed for reliable detection and analysis of target proteins. RPA2 is a key protein in DNA repair and replication processes, and its phosphorylation at these sites is essential for cell cycle progression and genome stability. This high-quality antibody, raised in rabbits, offers high reactivity with human samples and has been validated for use in Western blot applications. By binding to the phosphorylated forms of RPA2, this antibody allows for the precise detection and analysis of RPA2 phosphorylation in various cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
| Product Name: | Phospho-RPA2-S4/S8 Polyclonal Antibody |
| SKU: | CABP1102 |
| Size: | 20μL, 100μL |
| Reactivity: | Rat |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | Email for sequence | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | REPA2, RPA32, RP-A p32, RP-A p34, Phospho-RPA2-S4/S8 |
| Positive Sample: | C6 |
| Cellular Localization: | Nucleus, Pml Body. |
| Calculated MW: | 29kDa |
| Observed MW: | 36kDa |
This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.
| Purification Method | Affinity purification |
| Gene ID | 6118 |
| RRID | AB_2863971 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from C6 cells, using Phospho-RPA2-S4/S8 Rabbit pAb (CABP1102) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s. |
