The Phospho-SIRT1-S47 Monoclonal Antibody (CABP0976) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the phosphorylated form of Sirt1 at serine 47, making it ideal for investigating the regulation and function of this important protein.Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the phosphorylated serine 47 site on Sirt1, researchers can accurately detect and analyze the protein in various cell types, providing insights into its role in cellular signaling pathways and potential implications in diseases such as cancer, metabolic disorders, and neurodegenerative conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-SIRT1-S47 Monoclonal Antibody
SKU:
CABP0976
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC1520
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ERSP G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
23411
RRID
AB_2863872
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-SIRT1-S47 Rabbit mAb (CABP0976) at 1:500 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
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