The Phospho-Src-S17 Antibody (CABP0522) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, specifically binds to the phosphorylated form of Src at serine 17, allowing for the detection and analysis of this key regulatory protein. It is ideal for use in Western blot applications and is highly reactive with human samples.Src is a non-receptor tyrosine kinase that plays a crucial role in cell growth, proliferation, and survival. Phosphorylation of Src at serine 17 has been implicated in various cellular processes, including cell migration, invasion, and adhesion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Src-S17 Antibody
SKU:
CABP0522
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RRSL E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
6714
RRID
AB_2771554
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-Src-S17 Rabbit pAb (CABP0522) at 1:1000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
