Anti-Phospho-Src-Y419 Antibody (CABP1027)

SKU:
CABP1027
€129 - €389

Description

Technical Manual

Anti-Phospho-Src-Y419 Antibody

Product Name:Anti-Phospho-Src-Y419 Antibody
Product SKU:CABP1027
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Immunogen:A phospho specific peptide corresponding to residues surrounding Y419 of human Src
Sequence:

Email for sequence

Gene ID:6714
Swiss Prot:P12931
Synonyms:ASV; SRC1; THC6; c-SRC; p60-Src
Calculated MW:60kDa
Observed MW:60KDa
Reactivity:Human, Mouse
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:NIH/3T3
Cellular Location:

Product Images

Anti-Phospho-Src-Y419 Antibody (CABP1027)

Western blot - Phospho-Src-Y419 Rabbit mAb (CABP1027)

Uniprot Information

UniProt Protein Function:Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Oncoprotein; Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Src family

Chromosomal Location of Human Ortholog: 20q12-q13

Cellular Component: neuron projection; mitochondrion; lysosome; postsynaptic density; actin filament; caveola; cytosol; extrinsic to internal side of plasma membrane; perinuclear region of cytoplasm; late endosome; mitochondrial inner membrane; cytoplasm; plasma membrane; nucleus

Molecular Function:protein C-terminus binding; ephrin receptor binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; insulin receptor binding; protein kinase activity; integrin binding; protein binding; enzyme binding; SH3/SH2 adaptor activity; protein kinase C binding; protein-tyrosine kinase activity; heme binding; estrogen receptor binding; SH2 domain binding; kinase activity; ATP binding; receptor binding; hormone receptor binding

Biological Process: oogenesis; regulation of estrogen receptor signaling pathway; estrogen receptor signaling pathway; positive regulation of cyclin-dependent protein kinase activity; central nervous system development; progesterone receptor signaling pathway; nerve growth factor receptor signaling pathway; viral reproduction; regulation of cell cycle; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; uterus development; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; positive regulation of MAP kinase activity; cell-cell adhesion; transforming growth factor beta receptor signaling pathway; response to electrical stimulus; cell adhesion; bone resorption; response to drug; platelet activation; fibroblast growth factor receptor signaling pathway; activation of protein kinase B; response to virus; transcytosis; positive regulation of integrin activation; positive regulation of protein amino acid autophosphorylation; cellular response to insulin stimulus; response to mechanical stimulus; T cell costimulation; regulation of vascular permeability; negative regulation of transcription, DNA-dependent; leukocyte migration; negative regulation of apoptosis; axon guidance; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; platelet-derived growth factor receptor signaling pathway; negative regulation of caspase activity; signal transduction; positive regulation of smooth muscle cell migration; regulation of cell-cell adhesion; forebrain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; response to nutrient levels; regulation of bone resorption; negative regulation of focal adhesion formation; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; response to mineralocorticoid stimulus; cell cycle; regulation of cell proliferation; cell proliferation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; response to hydrogen peroxide; regulation of protein binding; Ras protein signal transduction; stress fiber formation; innate immune response; response to acidity; positive regulation of insulin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of cytokine secretion

NCBI Summary:This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P12931
NCBI GenInfo Identifier:125711
NCBI Gene ID:6714
NCBI Accession:P12931.3
UniProt Secondary Accession:P12931,Q76P87, Q86VB9, Q9H5A8, E1P5V4,
UniProt Related Accession:P12931
Molecular Weight:60kDa
NCBI Full Name:Proto-oncogene tyrosine-protein kinase Src
NCBI Synonym Full Names:SRC proto-oncogene, non-receptor tyrosine kinase
NCBI Official Symbol:SRC
NCBI Official Synonym Symbols:ASV; SRC1; c-SRC; p60-Src
NCBI Protein Information:proto-oncogene tyrosine-protein kinase Src; proto-oncogene c-Src; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; protooncogene SRC, Rous sarcoma; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
UniProt Protein Name:Proto-oncogene tyrosine-protein kinase Src
UniProt Synonym Protein Names:Proto-oncogene c-Src; pp60c-src; p60-Src
Protein Family:Proto-oncogene tyrosine-protein kinase
UniProt Gene Name:SRC
UniProt Entry Name:SRC_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
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