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Anti-Phospho-SRC-Y416 Antibody (CABP0480)

SKU:
CABP0480
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 16 Anti-Phospho-SRC-Y416 Antibody CABP0480
  • Cell Biology Antibodies 16 Anti-Phospho-SRC-Y416 Antibody CABP0480
€329 - €419
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Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Phospho-SRC-Y416 Antibody
Antibody SKU:CABP0480
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3
Immunogen:A phospho specific peptide corresponding to residues surrounding Y416 of human SRC Family
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:2534/3055/3932/4067/6714/7525
Uniprot:P06241/P08631/P06239/P07948/P12931/P07947
Cellular Location:Cell membrane, Cytoplasm, Mitochondrion inner membrane, Nucleus, cytoskeleton
Calculated MW:59kDa/60kDa
Observed MW:60kDa
Anti-Phospho-SRC-Y416 Antibody (CABP0480)Western blot analysis of extracts of NIH/3T3-Src cells, using Phospho-SRC Family-Y416 antibody (CABP0480). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Anti-Phospho-SRC-Y416 Antibody (CABP0480)Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SRC Family-Y416 pAb (CABP0480) at 1:1000 dilution or SRC antibody ( A18240).NIH/3T3 cells were treated by PDGF (100 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Synonyms:ASV, SRC1, THC6, c-SRC, p60-Src, SRC
Background:This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

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