The Phospho-Syk-Y323 Polyclonal Antibody (CABP0500) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, generated using the peptide containing phosphorylated tyrosine 323 of Syk, is highly specific and sensitive for detecting phosphorylation at this site. It is suitable for use in Western blot and immunohistochemistry applications, allowing for precise analysis of Syk activation in various cell types.Syk is a key signaling molecule involved in immune responses, particularly in B cell receptor signaling and the activation of immune cells. Phosphorylation of tyrosine 323 is a critical event in regulating Syk activity, making this antibody essential for studying the function of Syk in immune regulation and disease states.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Syk-Y323 Polyclonal Antibody
SKU:
CABP0500
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NPYE P
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IMD82, p72-Syk, Phospho-Syk-Y323
Positive Sample:
U-937 treated with ATP
Cellular Localization:
Cell Membrane, Cytoplasm, Cytosol.
Calculated MW:
72kDa
Observed MW:
72kDa
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6850
RRID
AB_2771586
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from U-937 cells using Phospho-Syk-Y323 Rabbit pAb (CABP0500) at 1:500 dilution. U-937 cells were treated with ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
