The Phospho-Tyrosine Hydroxylase-S40 Antibody (CABP1109) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Phospho-Tyrosine Hydroxylase-S40 Antibody
SKU:
CABP1109
Size:
100μL, 20μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Axon, Cytoplasm, Cytoplasmic Side Of Plasma Membrane, Cytoplasmic Vesicle, Cytosol, Dendrite, Mitochondrion, Neuron Projection, Nucleus, Perikaryon, Perinuclear Region Of Cytoplasm, Smooth Endoplasmic Reticulum, Synaptic Vesicle, Terminal Bouton.
Calculated MW:
45-59 kDa
Observed MW:
60 kDa
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Purification Method
Affinity purification
Gene ID
7054
RRID
AB_2863978
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-12 cells, using Phospho-Tyrosine Hydroxylase-S40 pAb (CABP1109) at 1:1000 dilution or Tyrosine Hydroxylase antibody (A0028). PC-12 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
