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Anti-Phospho-VIM-S39 pAb Antibody (CABP0806)

SKU:
CABP0806
Product Type:
Antibody
Applications:
WB
IHC
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 3 Anti-Phospho-VIM-S39 pAb Antibody CABP0806
  • Immunology Antibodies 3 Anti-Phospho-VIM-S39 pAb Antibody CABP0806
  • Immunology Antibodies 3 Anti-Phospho-VIM-S39 pAb Antibody CABP0806
€329 - €419
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Description

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Antibody Name:Anti-Phospho-VIM-S39 Antibody
Antibody SKU:CABP0806
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S39 of human VIM (NP_003371.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, NIH/3T3
Immunogen:A synthetic phosphorylated peptide around S39 of human VIM (NP_003371.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TYSL G
Gene ID:7431
Uniprot:P08670
Cellular Location:Cytoplasm
Calculated MW:53kDa
Observed MW:57KDa
Anti-Phospho-VIM-S39 pAb (CABP0806)Western blot analysis of extracts of NIH/3T3 cells, using Phospho-VIM-S39 antibody (CABP0806) at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37'C for 20 hours.NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00071). Exposure time: 1s.
Anti-Phospho-VIM-S39 pAb (CABP0806)Western blot analysis of extracts of HeLa cells, using Phospho-VIM-S39 antibody (CABP0806) at 1:1000 dilution.HeLa cells were treated by Paclitaxel (100 nM/ml) at 37'C for 20 hours.HeLa cells were treated by Hydroxyurea (4 mM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-Phospho-VIM-S39 pAb (CABP0806)Immunohistochemistry of paraffin-embedded human appendix using Phospho-VIM-S39 antibody (CABP0806) at dilution of 1:100 (40x lens).
Synonyms:CTRCT30, HEL113, Vimentin, VIM, vimentin
Background:This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
UniProt Protein Function:Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10p13

Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol

Molecular Function:protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens

Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; cell structure disassembly during apoptosis; muscle filament sliding

Disease: Cataract 30

NCBI Summary:This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017]
UniProt Code:P08670
NCBI GenInfo Identifier:55977767
NCBI Gene ID:7431
NCBI Accession:P08670.4
UniProt Related Accession:P08670
Molecular Weight:
NCBI Full Name:Vimentin
NCBI Synonym Full Names:vimentin
NCBI Official Symbol:VIM  
NCBI Protein Information:vimentin
UniProt Protein Name:Vimentin
Protein Family:Vimentin
UniProt Gene Name:VIM  
UniProt Entry Name:VIME_HUMAN

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