The PHOX2B Polyclonal Antibody (CAB21990) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity to human samples, making it ideal for Western blot applications. By targeting the Phox2B protein, this antibody allows for precise detection and analysis in various cell types, making it invaluable for investigations in neurobiology and developmental biology.Phox2B, a transcription factor essential for the proper formation of neurons in the autonomic nervous system, has been linked to disorders such as congenital central hypoventilation syndrome (CCHS).
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PHOX2B Polyclonal Antibody
SKU:
CAB21990
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CCHS, PMX2B, NBLST2, NBPhox, PHOX2B
Positive Sample:
Neuro-2a, SH-SY5Y
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
37kDa
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome.
Purification Method
Affinity purification
Gene ID
8929
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Neuro-2a cells using [KD Validated]PHOX2B Rabbit pAb (CAB21990) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time:10s.
Western blot analysis of lysates from wild type (WT) and PHOX2B knockdown (KD) SH-SY5Y cells using [KD Validated]PHOX2B Rabbit pAb (CAB21990) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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