Western blot analysis of various lysates, using PI3 Kinase p110 beta antibody (CAB22467) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The PI3 Kinase p110 beta Monoclonal Antibody (CAB22467) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced by AssayGenie, is monoclonal in nature, ensuring high specificity and sensitivity in experimental applications.The PI3 Kinase p110 Beta Monoclonal Antibody has been validated for use in various research applications, including Western blotting, immunoprecipitation, and immunofluorescence. It binds specifically to the p110 Beta subunit of PI3 Kinase, enabling researchers to detect and analyze protein expression levels in different cell types and tissues.The PI3 Kinase signaling pathway is known to play a crucial role in various cellular processes, including cell growth, proliferation, and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PI3 Kinase p110 beta Monoclonal Antibody
SKU:
CAB22467
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC54484
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5291
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-12 cells using PI3 Kinase p110 beta Rabbit mAb (CAB22467) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45 s.
Western blot analysis of lysates from 293F cells using PI3 Kinase p110 beta Rabbit mAb (CAB22467) at 1:10000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10 s.
![Anti- PI3 Kinase p110 beta [R03-5K7] Monoclonal Antibody - Knockout Validated (AGMB06612)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/277893/734883/anti-pi3-kinase-p110-beta-r03-5k7-monoclonal-antibody-knockout-validated-agmb06612__02953.1777194570.jpg?c=2 "Anti- PI3 Kinase p110 beta [R03-5K7] Monoclonal Antibody - Knockout Validated (AGMB06612)")
![Anti-PI3 Kinase p110 gamma [R09-5C8] Monoclonal Antibody (AGMB01346)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/images/stencil/590x590/products/272635/680211/anti-pi3-kinase-p110-gamma-r09-5c8-monoclonal-antibody-agmb01346__52531.1773041320.jpg?c=2 "Anti-PI3 Kinase p110 gamma [R09-5C8] Monoclonal Antibody (AGMB01346)")