Western blot analysis of various lysates, using PI3 Kinase p85 alpha/beta antibody (CAB22487) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The PI3 Kinase p85 alpha/beta Monoclonal Antibody (CAB22487) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, monoclonal in nature, specifically targets the p85 alpha/beta subunits of PI3 Kinase, which are crucial for its activation and function.The PI3 Kinase pathway is involved in a variety of cellular processes, including cell growth, proliferation, survival, and metabolism. Dysregulation of this pathway has been implicated in various diseases, including cancer, diabetes, and inflammatory disorders. The ability to specifically target the p85 alpha/beta subunits with this antibody allows for detailed analysis of their expression levels, localization, and post-translational modifications in different cell types and experimental conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PI3 Kinase p85 alpha/beta Monoclonal Antibody
SKU:
CAB22487
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC57210
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PIK3R1/PIK3R2, PI3 Kinase p85 alpha/beta
Positive Sample:
Jurkat, NIH/3T3
Cellular Localization:
Cell-Cell Junction, Cis-Golgi Network, Cytoplasm, Cytosol, Nucleus, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
42-54kDa/81-84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Purification Method
Affinity purification
Gene ID
5295 5296
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells using PI3 Kinase p85 alpha/beta Rabbit mAb (CAB22487) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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