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Anti-PIAS1 Antibody (CAB1007)

SKU:
CAB1007
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
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Description

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Antibody Name:Anti-PIAS1 Antibody
Antibody SKU:CAB1007
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 422-651 of human PIAS1 (NP_057250.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HeLa, A-549, Jurkat, HepG2, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 422-651 of human PIAS1 (NP_057250.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VQEV SASY NGVD GCLS STLE HQVA SHHQ SSNK NKKV EVID LTID SSSD EEEE EPSA KRTC PSLS PTSP LNNK GILS LPHQ ASPV SRTP SLPA VDTS YINT SLIQ DYRH PFHM TPMP YDLQ GLDF FPFL SGDN QHYN TSLL AAAA AAVS DDQD LLHS SRFF PYTS SQMF LDQL SAGG STSL PTTN GSSS GSNS SLVS SNSL RESH SHTV TNRS STDT ASIF GIIP DIIS LD
Gene ID:8554
Uniprot:O75925
Cellular Location:Nucleus, Nucleus speckle, PML body
Calculated MW:36kDa/71kDa/72kDa
Observed MW:72kDa
Synonyms:PIAS1, DDXBP1, GBP, GU/RH-II, ZMIZ3
Background:This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:PIAS1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Expressed in numerous tissues with highest level in testis. Belongs to the PIAS family.
UniProt Protein Details:

Protein type:EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q

Cellular Component: nucleoplasm; nucleus; PML body

Molecular Function:enzyme binding; protein binding; SUMO ligase activity; transcription corepressor activity

Biological Process: JAK-STAT cascade; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein sumoylation; protein sumoylation; regulation of cell proliferation

NCBI Summary:This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
UniProt Code:O75925
NCBI GenInfo Identifier:20138891
NCBI Gene ID:8554
NCBI Accession:O75925.2
UniProt Secondary Accession:O75925,Q147X4, Q99751, Q9UN02, B2RB67, B3KSY9, C5J4B4
UniProt Related Accession:O75925
Molecular Weight:36,204 Da
NCBI Full Name:E3 SUMO-protein ligase PIAS1
NCBI Synonym Full Names:protein inhibitor of activated STAT 1
NCBI Official Symbol:PIAS1  
NCBI Official Synonym Symbols:GBP; ZMIZ3; DDXBP1; GU/RH-II  
NCBI Protein Information:E3 SUMO-protein ligase PIAS1
UniProt Protein Name:E3 SUMO-protein ligase PIAS1
UniProt Synonym Protein Names:DEAD/H box-binding protein 1; Gu-binding protein; GBP; Protein inhibitor of activated STAT protein 1; RNA helicase II-binding protein
Protein Family:E3 SUMO-protein ligase
UniProt Gene Name:PIAS1  
UniProt Entry Name:PIAS1_HUMAN
Anti-PIAS1 Antibody (CAB1007)Western blot analysis of extracts of various cell lines, using PIAS1 antibody (CAB1007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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