Anti-PIP4K2B Antibody (CAB12535)

Antibody Name:	Anti-PIP4K2B Antibody
Antibody SKU:	CAB12535
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PIP4K2B (NP_003550.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, BT-474, A-549, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PIP4K2B (NP_003550.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSN CTST TAVA VAPL SASK TKTK KKHF VCQK VKLF RASE PILS VLMW GVNH TINE LSNV PVPV MLMP DDFK AYSK IKVD NHLF NKEN LPSR FKFK EYCP MVFR NLRE RFGI DDQD YQNS VTRS APIN SDSQ GRCG TRFL TTYD RRFV IKTV SSED VAEM HNIL KKYH QFIV ECHG NTLL PQFL GMYR LTVD GVET YMVV TRNV FSHR LTVH RKYD LKGS TVAR EASD KEKA KDLP TFKD NDFL NEGQ KLHV GEES KKNF LEKL KRDV EF
Gene ID:	8396
Uniprot:	P78356
Cellular Location:	Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Nucleus, Peripheral membrane protein
Calculated MW:	32kDa/47kDa
Observed MW:	47kDa

Synonyms:

PIP4K2B, PI5P4KB, PIP5K2B, PIP5KIIB, PIP5KIIbeta, PIP5P4KB

Background:

The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5, 4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor.

UniProt Protein Function:	PIP4K2B: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex. Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.149; Kinase, lipid; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 17q12 Cellular Component: autophagic vacuole; cytosol; nucleoplasm Molecular Function:1-phosphatidylinositol-4-phosphate 5-kinase activity; 1-phosphatidylinositol-5-phosphate 4-kinase activity; protein binding; protein homodimerization activity; receptor signaling protein activity Biological Process: cell surface receptor linked signal transduction; phosphatidylinositol biosynthetic process; phospholipid metabolic process; regulation of autophagy; regulation of phosphoinositide 3-kinase cascade
NCBI Summary:	The protein encoded by this gene catalyzes the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. The encoded protein sequence does not show similarity to other kinases, but the protein does exhibit kinase activity. Additionally, the encoded protein interacts with p55 TNF receptor. [provided by RefSeq, Jul 2008]
UniProt Code:	P78356
NCBI GenInfo Identifier:	47605991
NCBI Gene ID:	8396
NCBI Accession:	P78356.1
UniProt Secondary Accession:	P78356,Q5U0E8, Q8TBP2,
UniProt Related Accession:	P78356
Molecular Weight:	Calculated MW: 32kDa/47kDaObserved MW: 47kDa
NCBI Full Name:	Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
NCBI Synonym Full Names:	phosphatidylinositol-5-phosphate 4-kinase type 2 beta
NCBI Official Symbol:	PIP4K2B
NCBI Official Synonym Symbols:	PI5P4KB; PIP5K2B; PIP5KIIB; PIP5P4KB; PIP5KIIbeta
NCBI Protein Information:	phosphatidylinositol 5-phosphate 4-kinase type-2 beta
UniProt Protein Name:	Phosphatidylinositol 5-phosphate 4-kinase type-2 beta
UniProt Synonym Protein Names:	1-phosphatidylinositol 5-phosphate 4-kinase 2-beta; Diphosphoinositide kinase 2-beta; Phosphatidylinositol 5-phosphate 4-kinase type II beta; PI(5)P 4-kinase type II beta; PIP4KII-beta; PtdIns(5)P-4-kinase isoform 2-beta
Protein Family:	Phosphatidylinositol 5-phosphate 4-kinase
UniProt Gene Name:	PIP4K2B

	Western blot analysis of extracts of various cell lines, using PIP4K2B antibody (CAB12535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using PIP4K2B antibody (CAB12535). Blue: DAPI for nuclear staining.

Antibodies

Anti-PIP4K2B Antibody (CAB8016)

PIP4K2B Antibody (PACO11254)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)