Anti-PKLR Antibody (CAB1924)

Antibody Name:	Anti-PKLR Antibody
Antibody SKU:	CAB1924
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 375-574 of human PKLR (NP_000289.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 375-574 of human PKLR (NP_000289.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ESMI TKPR PTRA ETSD VANA VLDG ADCI MLSG ETAK GNFP VEAV KMQH AIAR EAEA AVYH RQLF EELR RAAP LSRD PTEV TAIG AVEA AFKC CAAA IIVL TTTG RSAQ LLSR YRPR AAVI AVTR SAQA ARQV HLCR GVFP LLYR EPPE AIWA DDVD RRVQ FGIE SGKL RGFL RVGD LVIV VTGW RPGS GYTN IMRV LSIS
Gene ID:	5313
Uniprot:	P30613
Cellular Location:
Calculated MW:	58kDa/61kDa
Observed MW:	62kDa

Synonyms:

PKLR, PK1, PKL, PKR, PKRL, RPK

Background:

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PKLR: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms have been described: the R- and L-types.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate; EC 2.7.1.40; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other Chromosomal Location of Human Ortholog: 1q21 Cellular Component: cytosol Molecular Function:pyruvate kinase activity Disease: Adenosine Triphosphate, Elevated, Of Erythrocytes; Pyruvate Kinase Deficiency Of Red Cells
NCBI Summary:	The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P30613
NCBI GenInfo Identifier:	8247933
NCBI Gene ID:	5313
NCBI Accession:	P30613.2
UniProt Secondary Accession:	P30613,O75758, P11973,
UniProt Related Accession:	P30613
Molecular Weight:	58,494 Da
NCBI Full Name:	Pyruvate kinase PKLR
NCBI Synonym Full Names:	pyruvate kinase, liver and RBC
NCBI Official Symbol:	PKLR
NCBI Official Synonym Symbols:	PK1; PKL; PKR; RPK; PKRL
NCBI Protein Information:	pyruvate kinase PKLR
UniProt Protein Name:	Pyruvate kinase PKLR
UniProt Synonym Protein Names:	Pyruvate kinase 1; Pyruvate kinase isozymes L/R; R-type/L-type pyruvate kinase; Red cell/liver pyruvate kinase
Protein Family:	Pyruvate kinase
UniProt Gene Name:	PKLR
UniProt Entry Name:	KPYR_HUMAN

	Western blot analysis of extracts of Raji cells, using PKLR antibody (CAB1924) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human normal breast using PKLR antibody (CAB1924) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human normal colon using PKLR antibody (CAB1924) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-PKLR Antibody (CAB12084)	Human PK (Pyruvate Kinase, Liver And RBC) CLIA Kit (HUES00756)
Anti-PKLR Antibody (CAB12336)	Human PK (Pyruvate Kinase, Liver And RBC) ELISA Kit (HUES02363)
PKLR Antibody (PACO11271)	Human Pyruvate kinase isozymes R/L (PKLR) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)