Western blot analysis of various lysates, using Phospho-PLC gamma 1 (PLCG1)-Y783 antibody (CABP1340) at1:2000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-PLC gamma 1 (PLCG1)-Y783 Monoclonal Antibody (CABP1340) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in mice, specifically targets the phosphorylated tyrosine 783 residue on the PLCG1 protein, a key regulator of signal transduction in cells.PLCG1 is involved in various cellular processes, including cell growth, differentiation, and immune responses. Phosphorylation of PLCG1 at Y783 is known to activate its enzymatic activity, leading to downstream signaling events. By specifically detecting this phosphorylated form of PLCG1, researchers can gain insights into how this protein contributes to signaling cascades in different cellular contexts.
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Western blot analysis of various lysates, using Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit mAb (CABP1340) at 1:2000 dilution. NIH/3T3 cells were treated with PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
