Anti-PLOD1 Antibody (CAB7919)

Antibody Name:	Anti-PLOD1 Antibody
Antibody SKU:	CAB7919
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 488-727 of human PLOD1 (NP_000293.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, NCI-H460, U-251MG, HepG2, Mouse brain, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 488-727 of human PLOD1 (NP_000293.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RQQD VFMF LTNR HTLG HLLS LDSY RTTH LHND LWEV FSNP EDWK EKYI HQNY TKAL AGKL VETP CPDV YWFP IFTE VACD ELVE EMEH FGQW SLGN NKDN RIQG GYEN VPTI DIHM NQIG FERE WHKF LLEY IAPM TEKL YPGY YTRA QFDL AFVV RYKP DEQP SLMP HHDA STFT INIA LNRV GVDY EGGG CRFL RYNC SIRA PRKG WTLM HPGR LTHY HEGL PTTR GTRY IAVS FVDP
Gene ID:	5351
Uniprot:	Q02809
Cellular Location:	Lumenal side, Peripheral membrane protein, Rough endoplasmic reticulum membrane
Calculated MW:	83kDa/88kDa
Observed MW:	110kDa

Synonyms:

PLOD1, EDS6, LH, LH1, LLH, PLOD

Background:

Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	PLOD1: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Defects in PLOD1 are the cause of Ehlers-Danlos syndrome type 6 (EDS6). EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS6 is characterized by the presence of ocular complications, particularly retinal detachment. Defects in PLOD1 are the cause of Nevo syndrome (NEVOS). This is a rare, autosomal recessive disorder characterized by increased perinatal length, kyphosis, muscular hypotonia, and joint laxity. Nevo syndrome and EDS-VI have similar clinical phenotypes. Some authors consider that both syndromes are the same clinical entity.
UniProt Protein Details:	Protein type:EC 1.14.11.4; Amino Acid Metabolism - lysine degradation; Oxidoreductase Chromosomal Location of Human Ortholog: 1p36.22 Cellular Component: endoplasmic reticulum membrane Molecular Function:procollagen-lysine 5-dioxygenase activity; protein homodimerization activity Biological Process: epidermis development; hydroxylysine biosynthetic process; response to hypoxia Disease: Ehlers-danlos Syndrome, Type Vi
NCBI Summary:	Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
UniProt Code:	Q02809
NCBI GenInfo Identifier:	78099790
NCBI Gene ID:	5351
NCBI Accession:	Q02809.2
UniProt Secondary Accession:	Q02809,Q96AV9, Q9H132, B4DR87,
UniProt Related Accession:	Q02809
Molecular Weight:	88,273 Da
NCBI Full Name:	Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
NCBI Synonym Full Names:	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
NCBI Official Symbol:	PLOD1
NCBI Official Synonym Symbols:	LH; LH1; LLH; EDS6; PLOD
NCBI Protein Information:	procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Protein Name:	Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
UniProt Synonym Protein Names:	Lysyl hydroxylase 1; LH1
Protein Family:	Procollagen-lysine,2-oxoglutarate 5-dioxygenase
UniProt Gene Name:	PLOD1
UniProt Entry Name:	PLOD1_HUMAN

	Western blot analysis of extracts of various cell lines, using PLOD1 antibody (CAB7919) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s.
	Immunofluorescence analysis of U2OS cells using PLOD1 antibody (CAB7919) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-PLOD1 Antibody (CAB14044)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)