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Anti-PMAIP1 Antibody (CAB9801)

SKU:
CAB9801
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
  • Cell Death Antibodies 2 Anti-PMAIP1 Antibody CAB9801
  • Cell Death Antibodies 2 Anti-PMAIP1 Antibody CAB9801
  • Cell Death Antibodies 2 Anti-PMAIP1 Antibody CAB9801
  • Cell Death Antibodies 2 Anti-PMAIP1 Antibody CAB9801
€139 - €419
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Description

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Antibody Name:Anti-PMAIP1 Antibody
Antibody SKU:CAB9801
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-54 of human PMAIP1 (NP_066950.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HeLa, HT-1080, SKOV3, BT-474
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-54 of human PMAIP1 (NP_066950.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPGK KARK NAQP SPAR APAE LEVE CATQ LRRF GDKL NFRQ KLLN LISK LFCS GT
Gene ID:5366
Uniprot:Q13794
Cellular Location:Mitochondrion
Calculated MW:6kDa/14kDa
Observed MW:14kDa
Synonyms:PMAIP1, APR, NOXA
Background:
UniProt Protein Function:NOXA: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1. Interacts with MCL1, BCL2A1 and BAX. Up-regulated by p53/TP53, phorbol esters, double- stranded RNA, IFNB1/IFN-beta and viruses. Highly expressed in adult T-cell leukemia cell line. Belongs to the PMAIP1 family.
UniProt Protein Details:

Protein type:Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 18q21.32

Cellular Component: cytosol; mitochondrial outer membrane; mitochondrion; nucleus

Molecular Function:protein binding

Biological Process: apoptosis; cellular response to glucose starvation; defense response to virus; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of protein oligomerization; proteasomal protein catabolic process; protein insertion into mitochondrial membrane during induction of apoptosis; regulation of apoptosis; regulation of mitochondrial membrane permeability; response to dsRNA; T cell homeostasis

UniProt Code:Q13794
NCBI GenInfo Identifier:6919841
NCBI Gene ID:5366
NCBI Accession:Q13794.1
UniProt Secondary Accession:Q13794,Q8N589, B2R4T7,
UniProt Related Accession:Q13794
Molecular Weight:6kDa
NCBI Full Name:Phorbol-12-myristate-13-acetate-induced protein 1
NCBI Synonym Full Names:phorbol-12-myristate-13-acetate-induced protein 1
NCBI Official Symbol:PMAIP1  
NCBI Official Synonym Symbols:APR; NOXA  
NCBI Protein Information:phorbol-12-myristate-13-acetate-induced protein 1
UniProt Protein Name:Phorbol-12-myristate-13-acetate-induced protein 1
UniProt Synonym Protein Names:Immediate-early-response protein APR; Protein Noxa
Protein Family:Phorbol-12-myristate-13-acetate-induced protein
UniProt Gene Name:PMAIP1  
Anti-PMAIP1 Polyclonal Antibody (CAB9801)Western blot analysis of extracts of various cell lines, using PMAIP1 antibody (CAB9801) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-PMAIP1 Polyclonal Antibody (CAB9801)Immunofluorescence analysis of Jurkat cells using PMAIP1 antibody (CAB9801) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-PMAIP1 Polyclonal Antibody (CAB9801)Immunofluorescence analysis of rat thymus cells using PMAIP1 antibody (CAB9801) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-PMAIP1 Polyclonal Antibody (CAB9801)Immunofluorescence analysis of mouse thymus cells using PMAIP1 antibody (CAB9801) at dilution of 1:100. Blue: DAPI for nuclear staining.

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