Anti-PMEPA1 Antibody (CAB12171)

Antibody Name:	Anti-PMEPA1 Antibody
Antibody SKU:	CAB12171
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 173-252 of human PMEPA1 (NP_954638.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, 22Rv1, 293T, Mouse brain, Rat brain, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 173-252 of human PMEPA1 (NP_954638.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PSSN SGIS ATCY GSGG RMEG PPPT YSEV IGHY PGSS FQHQ QSSG PPSL LEGT RLHH THIA PLES AAIW SKEK DKQK GHPL
Gene ID:	56937
Uniprot:	Q969W9
Cellular Location:	Early endosome membrane, Golgi apparatus membrane, Single-pass membrane protein
Calculated MW:	26kDa/27kDa/31kDa
Observed MW:	37kDa

Synonyms:

PMEPA1, STAG1, TMEPAI

Background:

This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	TMEPAI: Involved in down-regulation of the androgen receptor (AR). Enhances ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4. Belongs to the PMEPA1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 20q13.31 Cellular Component: early endosome membrane; endosome membrane; Golgi membrane; intracellular membrane-bound organelle; plasma membrane Molecular Function:protein binding; WW domain binding Biological Process: negative regulation of transforming growth factor beta receptor signaling pathway
NCBI Summary:	This gene encodes a transmembrane protein that contains a Smad interacting motif (SIM). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins. Overexpression of this gene may play a role in multiple types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	Q969W9
NCBI GenInfo Identifier:	20140695
NCBI Gene ID:	56937
NCBI Accession:	Q969W9.1
UniProt Secondary Accession:	Q969W9,Q5TDR6, Q8NER4, Q96B72, Q9UJD3,
UniProt Related Accession:	Q969W9
Molecular Weight:	32kDa
NCBI Full Name:	Protein TMEPAI
NCBI Synonym Full Names:	prostate transmembrane protein, androgen induced 1
NCBI Official Symbol:	PMEPA1
NCBI Official Synonym Symbols:	STAG1; TMEPAI
NCBI Protein Information:	protein TMEPAI
UniProt Protein Name:	Protein TMEPAI
UniProt Synonym Protein Names:	Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein
Protein Family:	Protein
UniProt Gene Name:	PMEPA1

	Western blot analysis of extracts of various cell lines, using PMEPA1 antibody (CAB12171) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using PMEPA1 antibody (CAB12171) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-PMEPA1 Antibody (CAB16555)

PMEPA1 Antibody (PACO06769)

PMEPA1 Antibody (PACO07321)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)