The PNO1 Antibody (CAB17736) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables RNA binding activity. Located in nucleolus and nucleoplasm.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
PNO1 Antibody
SKU:
CAB17736
Size:
100μL, 20μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RRP20, KHRBP1, PNO1
Positive Sample:
A-549, MCF7, LO2
Cellular Localization:
Cytosol, Nucleolus, Nucleoplasm.
Calculated MW:
28kDa
Observed MW:
28kDa
Enables RNA binding activity. Located in nucleolus and nucleoplasm.
Purification Method
Affinity purification
Gene ID
56902
RRID
AB_2771729
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PNO1 Rabbit pAb (CAB17736) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
