The POLE4 Polyclonal Antibody (CAB24516) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific for detecting Pole4 in human samples and has been validated for use in Western blot applications. By binding to the Pole4 protein, researchers can easily visualize and analyze its expression in various cell types, making it an essential tool for studies in genetics and cancer biology.Pole4 is essential for maintaining genomic stability and proper functioning of the cell cycle, making it a crucial target for research into cancer development and progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POLE4 Polyclonal Antibody
SKU:
CAB24516
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
POLE4, YHHQ1, p12, DNA polymerase epsilon subunit 4
Positive Sample:
Mouse liver
Cellular Localization:
Nucleus.
Calculated MW:
12kDa
Observed MW:
15kDa
POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004]
Purification Method
Affinity purification
Gene ID
56655
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse liver, using POLE4 Rabbit pAb (CAB24516) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
