The POLR1B Antibody (CAB12034) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunohistochemistry applications.POLR1B is a subunit of RNA polymerase I, which is responsible for transcribing ribosomal RNA genes. This enzyme plays a crucial role in the regulation of protein synthesis and cell growth. The POLR1B antibody enables detection and analysis of POLR1B protein levels in various cell types, making it an essential tool for studies in molecular biology, cell biology, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POLR1B Antibody
SKU:
CAB12034
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
A135, RPA2, TCS4, RPA135, Rpo1-2, POLR1B
Positive Sample:
HeLa, HT-29, 293T, Mouse spleen
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
128kDa
Observed MW:
128kDa
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).
Purification Method
Affinity purification
Gene ID
84172
RRID
AB_2758945
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POLR1B Rabbit pAb (CAB12034) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
