The POLR2I Antibody (CAB1865) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. It binds specifically to the POLR2I protein, allowing for accurate detection and analysis in a variety of cell types.POLR2I is essential for the transcription of protein-coding genes and is involved in various biological processes, including cell growth and differentiation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
POLR2I Antibody
SKU:
CAB1865
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
MEPD GTYE PGFV GIRF CQEC NNML YPKE DKEN RILL YACR NCDY QQEA DNSC IYVN KITH EVDE LTQI IADV SQDP TLPR TEDH PCQK CGHK EAVF FQSH SARA EDAM RLYY VCTA PHCG HRWT E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPB9, hRPB14.5, POLR2I
Positive Sample:
MCF7, SW480, HL-60, 22Rv1
Cellular Localization:
Nucleus, Nucleolus.
Calculated MW:
15kDa
Observed MW:
15kDa
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.
Purification Method
Affinity purification
Gene ID
5438
RRID
AB_2763899
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using POLR2I Rabbit pAb (CAB1865) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
