Anti-PON1 Antibody (CAB7338)

Antibody Name:	Anti-PON1 Antibody
Antibody SKU:	CAB7338
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human PON1 (NP_000437.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, Jurkat, Mouse thymus, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 20-150 of human PON1 (NP_000437.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HQSS YQTR LNAL REVQ PVEL PNCN LVKG IETG SEDL EILP NGLA FISS GLKY PGIK SFNP NSPG KILL MDLN EEDP TVLE LGIT GSKF DVSS FNPH GIST FTDE DNAM YLLV VNHP DAKS TVEL FKFQ EEE
Gene ID:	5444
Uniprot:	P27169
Cellular Location:	Secreted, extracellular space
Calculated MW:	39kDa
Observed MW:	40kDa/55kDa

Synonyms:	PON1, ESA, MVCD5, PON
Background:	The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3.

UniProt Protein Function:	PON1: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. Genetic variation in PON1 is associated with susceptibility to microvascular complications of diabetes type 5 (MVCD5). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Homozygosity for the Leu-54 allele is strongly associated with the development of retinal disease in diabetic patients. Belongs to the paraoxonase family.
UniProt Protein Details:	Protein type:Lipid-binding; EC 3.1.8.1; EC 3.1.1.2; Secreted, signal peptide; Hydrolase; Secreted; Phosphatase (non-protein); Motility/polarity/chemotaxis; EC 3.1.1.81 Chromosomal Location of Human Ortholog: 7q21.3 Cellular Component: extracellular space; intracellular membrane-bound organelle; extracellular region Molecular Function:protein homodimerization activity; arylesterase activity; phospholipid binding; calcium ion binding; aryldialkylphosphatase activity Biological Process: response to nutrient levels; response to external stimulus; dephosphorylation; organophosphate catabolic process; response to toxin; positive regulation of transporter activity; carboxylic acid catabolic process; positive regulation of binding; aromatic compound catabolic process; phosphatidylcholine metabolic process Disease: Microvascular Complications Of Diabetes, Susceptibility To, 5
NCBI Summary:	The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008]
UniProt Code:	P27169
NCBI GenInfo Identifier:	308153572
NCBI Gene ID:	5444
NCBI Accession:	P27169.3
UniProt Secondary Accession:	P27169,Q16052, Q6B0J6, Q9UCB1, B2RA40,
UniProt Related Accession:	P27169
Molecular Weight:	39,731 Da
NCBI Full Name:	Serum paraoxonase/arylesterase 1
NCBI Synonym Full Names:	paraoxonase 1
NCBI Official Symbol:	PON1
NCBI Official Synonym Symbols:	ESA; PON; MVCD5
NCBI Protein Information:	serum paraoxonase/arylesterase 1; K-45; PON 1; esterase A; A-esterase 1; paraoxonase B-type; aromatic esterase 1; arylesterase B-type; serum aryldiakylphosphatase; serum aryldialkylphosphatase 1
UniProt Protein Name:	Serum paraoxonase/arylesterase 1
UniProt Synonym Protein Names:	Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1
Protein Family:	Serum paraoxonase/arylesterase
UniProt Gene Name:	PON1
UniProt Entry Name:	PON1_HUMAN

	Western blot analysis of extracts of various cell lines, using PON1 antibody (CAB7338) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver cancer using PON1 antibody (CAB7338) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-PON1 Antibody (CAB1321)	Human PON1 (Paraoxonase 1) CLIA Kit (HUES01194)
PON1 Antibody (PACO11369)	Human Serum paraoxonase/arylesterase 1 (PON1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)