The PON3 Antibody (CAB16418) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and reactivity towards human samples, making it ideal for use in Western blot applications.PON3, part of the paraoxonase family of enzymes, plays a crucial role in protecting against oxidative stress and lipid peroxidation, contributing to overall cardiovascular health. Dysregulation of PON3 has been linked to various diseases, including atherosclerosis and cardiovascular disorders, highlighting the importance of studying this enzyme in medical research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PON3 Antibody
SKU:
CAB16418
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PON3
Positive Sample:
Jurkat, HepG2
Cellular Localization:
Secreted, Extracellular Space.
Calculated MW:
40kDa
Observed MW:
39kDa
This gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein (LDL), a function that is believed to slow the initiation and progression of atherosclerosis. Alternatively spliced variants which encode different protein isoforms have been described; however, only one has been fully characterized.
Purification Method
Affinity purification
Gene ID
5446
RRID
AB_2771749
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PON3 Rabbit pAb (CAB16418) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
