The Porcine Diamine Oxidase (DAO) ELISA Kit is a powerful tool for the quantitative measurement of DAO levels in porcine samples, including serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, delivering accurate and consistent results for research applications.DAO is an enzyme that plays a key role in the metabolism of histamine, which is involved in various physiological and pathological processes. Abnormal DAO levels have been linked to conditions such as food intolerance, gastrointestinal disorders, and histamine intolerance, highlighting the importance of studying this enzyme in research and clinical settings.
With its reliable performance and user-friendly format, the Porcine DAO ELISA Kit is an essential resource for investigating the role of DAO in health and disease, offering valuable insights for potential therapeutic interventions.
Product Name:
Porcine Diamine Oxidase (DAO) ELISA Kit
Product Code:
PRDL00039
Size:
96 Assays
Target:
DAO
Synonyms:
AOC1, ABP1, KAO, Histaminase, Kidney amine oxidase, Amiloride Binding Protein 1, Amiloride-sensitive amine oxidase, Amine oxidase copper domain-containing protein 1
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
