The Porcine HGF (Hepatocyte Growth Factor) ELISA Kit is a powerful tool for the precise quantification of porcine HGF levels in biological samples such as serum, plasma, and tissue culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring reliable and consistent results for various research applications. HGF, also known as scatter factor, is a key growth factor that plays a critical role in tissue regeneration, wound healing, and organ development. It is particularly important in liver regeneration and has been implicated in various disease processes, including liver fibrosis, cancer, and cardiovascular diseases.
As such, HGF serves as a valuable biomarker for studying these conditions and exploring potential treatment strategies. With the Porcine HGF ELISA Kit, researchers can accurately measure HGF levels in porcine samples, providing valuable insights into the mechanisms underlying these biological processes. This kit is user-friendly, efficient, and designed to deliver precise and dependable results, making it an indispensable tool for advancing scientific knowledge and therapeutic discoveries in the field of HGF research.
Product Name:
Porcine HGF (Hepatocyte Growth Factor) ELISA Kit
SKU:
PRFI00206
Reactivity:
Porcine
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
28.125 pg/mL
Range:
46.875-3000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
84-100%
82-100%
84-98%
EDTA Plasma (n = 5)
85-94%
82-100%
84-98%
Heparin Plasma (n = 5)
91-94%
81-99%
82-100%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-103
95
EDTA Plasma (n = 5)
85-105
95
Heparin Plasma (n = 5)
89-105
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
